cysL [2017-8-11 16:0:3]

transcriptional activator of the cysJ-cysI operon

locus: BSU37650

pI: 6.96

mw: 34.04 kDa

protein length: 299 aa

gene length: 897 bp

function: regulation of cysteine biosynthesis

product: transcriptional regulator (LysR family)

essential: no

synonyms: ywfK, ipa-89d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 3,864,309 → 3,865,208

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source [Pubmed|12169591]

The protein

Protein family

• [SW|LysR family]

Expression and Regulation

Operons

• genes: [gene|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|cysL]
  description: [Pubmed|12169591]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12169591], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL]: auto-repression, [Pubmed|12169591], in [regulon|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL regulon]

  regulation

  • repressed by sulfate, sulfite, and thiosulfate ([protein|search|CysL]) [Pubmed|12169591]

Biological materials

Mutant

• MGNA-B668 (ywfK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1667 NBRP B. subtilis, Japan]

References

• 12169591